This is the laboratory course that provides students with training on RDT tools. After this course, the students develop the skills and understand the basic workings of recombinant DNA technology. The course provides handling the DNA, estimation of its purity and removing the impurities, cutting with restriction enzymes, transformation, etc.

CO1

Acquire a comprehensive knowledge of history, scope of Genetic Engineering and understand Biosafety and Ethics in Biotechnology.

CO2

Understand and analyse different tools of RDT in gene cloning and expression.

CO3

Apply the knowledge of different tools in cloning and expression of genes.

CO4

Analyse and evaluate nucleic acid and protein molecules.

CO5

Design and develop assays and protocols for recombinant therapeutics transgenics and other applications.

Course Outcomes

CO1

Understand the concepts of media design, optimization & sterilization.

CO2

Learn the microbial growth kinetics in various culture modes.

CO3

Analyse the concept of solid-state fermentations and immobilized cell

systems.

CO4

Understand the operation and optimization of process parameters.

CO5

Understand the processing of several industrial bioproducts.